April 21, 2010: Lecture on Transgenic Mice, Knockout Mice, and Genetically-Modified Foods
After this lecture, you should be able to answer the following:1. What is the difference between a transgenic mouse and a knockout mouse? How can such tools be used to answer experimental questions relating to human disease states?
2. You have generated a chimeric knockout mouse using embryonic stem cells. Describe how you did it. What were some of the features of the vector that you used? You thought that knocking out this gene would produce an embryonic lethal phenotype, but it did not. To your surprise, the mouse survived and did not appear to have any phenotypic abnormalities. What do you conclude?
3. How could you make a transgenic mouse which will express the transgene in a tissue-specific manner, such as in the prostate, rather than throughout the body?
HINT: This web-site has some helpful information:
http://www.biochem.arizona.edu/classes/bioc471/pages/Lecture19/Lecture19.html
4. What kinds of crops have been genetically modified using biotechnologies? What percentage of the biotech food market is accounted for by the USA and Canada? By China? What percentage of the soy beans sold in the USA are genetically-modified?
5. What are some of the advantages and disadvantages of genetically-modified foods?
posted by Blase Billack, Ph.D. @ 5:32 AM
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