Lecture 4: DNA Replication, Mutation, and Repair
Students: Our class is cancelled. But I have emailed you the slide set. Please read it and answer the questions below. Note that our next meeting is February 24th!Try to get through your Background and Significance by then. On the 24th, I will explain the Preliminary Studies section. Good luck!
After reading this slide set, you should be able to answer the following:
1. Describe DNA replication in E. coli. Where does replication begin? Anywhere on the chromosome or at a specific site? How many such sites are present on the E. coli chromosome? How many such sites are present in human chromosomes? What are the proteins involved in DNA replication in E. coli (name the protein and its function)? What are "replication bubbles?" What are "replication forks?" What do we mean when we say that a DNA polymerase has proofreading activity?
2. Describe the different types of gene mutations.
3. What is the AMES test? what is the principle behind the test?
4. What is mismatch repair? What are the different types of mismatch repair? What are the enzymes involved in these two types of mismatch repair?
posted by Blase Billack, Ph.D. @ 7:00 PM
0 Comments:
Post a Comment
<< Home